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| Registros recuperados: 31 | |
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| TAGAWA, Michihito; TAKEUCHI, Toshihiko; FUJISAWA, Tetsuro; KONNO, Yasuhiro; YAMAMOTO, Shinji; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| A 2-year-old East Friesian sheep imported from Australia exhibited severe anemia after contagious pustular dermatitis in Hokkaido, Japan. Hemoplasma infection was confirmed in blood smears. Both Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’ were detected by PCR and sequence analyses. In the epidemiological analysis, dual pathogens were detected in 6 of 12 (50.0%) sheep imported from Australia with the infected ewe at the same time, 1 of 5 (20.0%) sheep introduced from a domestic farm in Hokkaido, and in 1 of 16 (6.3%) sheep from an epidemiologically unrelated ranch. It is the first clinical case of sheep to confirm coinfection of these pathogens in Japan. |
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Palavras-chave: 'Candidatus Mycoplasma haemovis'; Case report; Japan; Mycoplasma ovis; Sheep. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3582 |
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| 田川, 道人; 千葉, 史織; 岡松, 弘之; 小嶋, 由夏; 松本, 高太郎; 古林, 与志安; 猪熊, 壽; MATSUMOTO, Kotaro; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi. |
| 黒毛和種牛が出生翌日より横臥，後弓反張を呈した．脳挫傷を疑い，抗生物質，ビタミンB1製剤，消炎鎮痛剤により加療され，いったんは改善したが同様の症状が間欠的に認められた．歩様異常は継続して認められたが，一般状態は良好で8カ月齢まで飼養された．しかし，その後開脚姿勢，測定過大を伴う歩様異常，威嚇瞬き反応の低下が進行した．病理組織学的検索により小脳にプルキンエ細胞の顕著な変性と脱落及び顆粒細胞の減数を認め，小脳皮質変性症と診断された． |
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Palavras-chave: 牛; 小脳皮質変性症; 黒毛和種. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4101 |
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| MATSUMOTO, Kotaro; TAKEUCHI, Toshihiko; YOKOYAMA, Naoaki; KATAGIRI, Yoshito; OOSHIRO, Mamoru; ZAKIMI, Satoshi; Gaowa; KAWAMORI, Fumihiko; OHASHI, Norio; INOKUMA, Hisashi. |
| We collected a total of 206 Haemaphysalis longicornis ticks by flagging in pastures in Yonaguni Island, Okinawa, Japan, in April 2008. Four of the 206 tick DNA samples tested were positive in a polymerase chain reaction (PCR) screening for the 16SrRNA gene of Anaplasmataceae. Partial sequences of 4 PCR products were identical to each other. Longer sequences of the 16SrRNA gene were successfully determined in 2 of the 4 tick samples, and the obtained 1,392 bp and 1,300 bp sequences revealed high similarity to the 16SrRNA gene sequences of the validated Ehrlichia species, including Ehrlichia ewingii, E. chaffeensis, and E. canis (98.3–98.6%). We also sequenced 1,304 bp of the groEL gene from the 2 tick samples, and found that these had the highest similarity... |
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Palavras-chave: 16SrRNA; Ehrlichia; GroEL; Haemaphysalis longicornis. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3581 |
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| HORIUCHI, Noriyuki; KUMAGAI, Daishiro; MATSUMOTO, Kotaro; INOKUMA, Hisashi; FURUOKA, Hidefumi; KOBAYASHI, Yoshiyasu; 松本, 高太郎; 猪熊, 壽; 古岡, 秀文; 古林, 与志安. |
| Bovine dilated cardiomyopathy (DCM) is an autosomal recessive genetic disorder causing congestive heart failure and subsequent death. Recently, a nonsense mutation c.343C>T in the bovine optic atrophy 3 (OPA3) gene had been reported to cause the DCM in Holstein cattle in Switzerland. However, the mutation has not been confirmed in bovine DCM outside Switzerland. Nine Holstein Friesian cows that were macroscopically and histologically diagnosed with or suspected of DCM and 12 control cows kept in Japan were tested for the mutation. The mutation surrounding OPA3 DNA fragment was amplified by PCR and subjected to direct sequences. The homogeneous c.343C>T mutation was proved to occur in all the affected cows and not in the control cows. The present... |
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Palavras-chave: Cattle; Dilated cardiomyopathy; Nonsense mutation; OPA3 gene. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4208 |
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| YBAÑEZ, Adrian Patalinghug; MATSUMOTO, Kotaro; KISHIMOTO, Toshio; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| Anaplasma phagocytophilum causes human granulocytic anaplasmosis (HGA) and tick-borne fever in ruminants. A closely related and potentially novel Anaplasma sp. in Japan was recently characterized. The aims of the study were to provide molecular evidence for the presence of these 2 species in Japan, and to develop a reliable PCR method based on the nucleotide differences within the citrate synthase (gltA) gene. DNA samples from 182 ixodid ticks (134 Ixodes persulcatus, 35 Haemaphysalis douglasii and 13 I. ovatus) collected from 2 sites in Hokkaido, Japan, were screened for A. phagocytophilum and its closely related Anaplasma sp. (herein designated as Anaplasma sp. Japan) using 16S rRNA PCR, revealing a combined prevalence rate of 27.5% (50 samples). The... |
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Palavras-chave: 16S rRNA; Anaplasma phagocytophilum; Anaplasma sp.; GltA; GroEL. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3839 |
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| TAGAWA, Michihito; YAMAKAWA, Kazuhiro; AOKI, Takahiro; MATSUMOTO, Kotaro; ISHII, Mitsuo; INOKUMA, Hisashi; 滄木, 孝弘; 松本, 高太郎; 石井, 三都夫; 猪熊, 壽. |
| The present study evaluated the effect of hemoplasmosis on cattle productivity. Prevalence of bovine hemoplasma was examined by polymerase chain reaction (PCR) using whole blood samples collected from 93 breeding cows and their 71 calves in Hokkaido, Japan. Monthly milk production records and other clinical data were compared between Mycoplasma wenyonii (Mw)-infected, “Candidatus Mycoplasma haemobos” (CMh)-infected, co-infected and PCR-negative groups. Blood chemical parameters were obtained from the 93 cows and 64 calves. PCR results showed that 89.2% (83/93) of cows and 14.1% (10/71) of calves were positive for bovine hemoplasma. Based on productivity data obtained from the 93 cows, Mw-infected, CMh-infected and co-infected cows had significantly lower... |
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Palavras-chave: Cattle; Direct PCR; Hemoplasma; Productivity. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3870 |
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| YBAÑEZ, Adrian Patalinghug; SIVAKUMAR, Thillaiampalam; YBAÑEZ, Rochelle Haidee Daclan; RATILLA, Jowarren Catingan; PEREZ, Zandro Obligado; GABOTERO, Shirleny Reyes; HAKIMI, Hassan; KAWAZU, Shin-ichiro; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 河津, 信一郎; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| Anaplasma marginale has been detected in the Philippines only by peripheral blood smear examination and serological methods. This study generally aimed to molecularly detect and characterize A. marginale in cattle and ticks in Cebu, Philippines. A total of 12 bovine blood samples and 60 Rhipicephalus (Boophilus) microplus ticks were collected on the Cebu Island in 2011. 16S rRNA-based screening-PCR and DNA sequencing revealed 8 cattle (66.7%) and 8 ticks (13.3%) to be positive for A. marginale, and 1 tick (1.7%) to be positive for A. centrale. Selected positive DNA samples were further characterized based on 16S rRNA (longer sequence), Msp5, Msp1α,gltA and groEL genes for phylogenetic analyses. Sequence identities of partial DNA fragments of A. marginale... |
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Palavras-chave: Anaplasma marginale; Cattle; PCR; Philippines; Rhipicephalus (Boophilus) microplus. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3866 |
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| YOKOYAMA, Naoaki; UENO, Akio; MIZUNO, Daisuke; KUBOKI, Noritaka; KHUKHUU, Altangerel; IGARASHI, Ikuo; MIYAHARA, Tokuji; SHIRAISHI, Takashi; KUDO, Ryuta; OSHIRO, Mamoru; ZAKIMI, Satoshi; SUGIMOTO, Chihiro; MATSUMOTO, Kotaro; INOKUMA, Hisashi; 横山, 直明; 五十嵐, 郁男; 松本, 高太郎; 猪熊, 壽. |
| Theileria orientalis is a benign protozoan species that is widely distributed in Japan, yet sometimes causes serious economic losses in the livestock industry. In this study, we conducted a molecular survey based on genes encoding the major piroplasm surface protein (MPSP) and p23 for T. orientalis detected in cattle grazing in southern areas of Japan, consisting of 2 farms in Kumamoto prefecture (Aso and Kuma districts) and 3 farms in Okinawa prefecture (Ishigaki, Iriomote, and Yonaguni Islands). High prevalence rates of T. orientalis infection were shown in all the cattle populations using the diagnostic MPSP- and p23-PCR assays. Phylogenetic analyses revealed 4 MPSP genotypes and 3 p23 genotypes. Furthermore, MPSP genotype-specific PCR methods were... |
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Palavras-chave: Cattle; Genotype-specific PCR; Japan; MPSP gene; Theileria orientalis. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3584 |
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| HORIUCHI, Noriyuki; KOMAGATA, Makoto; SHITAMURA, Keiichi; CHIBA, Shiori; MATSUMOTO, Kotaro; INOKUMA, Hisashi; MATSUI, Takane; KOBAYASHI, Yoshiyasu; 堀内, 雅之; 松本, 高太郎; 猪熊, 壽; 古林, 与志安. |
| An 11-year-old Holstein-Friesian cow exhibited anorexia and jaundice. A large mass was found in the liver during necropsy. Macroscopically, the mass was composed of dark red multilobular tissue and a centrally located abscess, which was connected to the hepatic duct. Histologically, the mass consisted of proliferation of small neoplastic cells and was demarcated from the hepatic parenchyma by a thick region of granulation tissue. The neoplastic cells were predominantly arranged in solid sheets, but they also formed blood-filled cancellous structures, and proliferating foci were seen around blood vessels. Periodic acid-Schiff reaction demonstrated that a fine basement membrane-like structure surrounded the neoplastic cells. Immunohistochemically, the... |
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Palavras-chave: Cholangitic abscess; Cow; Glomus tumor. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4172 |
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| 田川, 道人; 大橋, 英二; 本村, 絹代; 千葉, 史織; 堀内, 雅之; 三好, 雅史; 猪熊, 壽; 松本, 高太郎; TAGAWA, Michihito; OHASHI, Eiji; MOTOMURA, Kinuyo; CHIBA, Shiori; HORIUCHI, Noriyuki; MIYOSHI, Masahumi; INOKUMA, Hisashi; MATSUMOTO, Kotaro. |
| 10歳の雄の雑種犬が，3週間前から持続する発熱及び元気消失を主訴に帯広畜産大学動物医療センターを受診した．心雑音及び心エコー検査において僧帽弁の疣贅物が確認され，抗生物質等により加療したが，第7病日より心不全の悪化を認め，第10病日に肺水腫により斃死した．病理解剖では，軽度の心拡大及び僧帽弁における多中心性疣贅物が認められた．血液培養検査では，Staphylococcus haemolyticusが検出されたが，疣贅物を用いたPCR検査ではStreptococcus canisと100％一致する塩基配列が得られた．血液培養分離菌はコンタミネーションであった可能性が高く，本症例の心内膜炎はStreptococcus canisによるものと考えられた．血液培養結果と実際の心内膜炎起因菌が異なる場合があることを考慮する必要がある． |
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Palavras-chave: 心内膜炎; PCR; Streptococcus canis. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4232 |
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| KHUKHUU, Altangerel; LAN, Dinh Thi Bich; LONG, Phung Thang; UENO, Akio; LI, Yan; LUO, Yuzi; MACEDO, Alan Caine Costa de; MATSUMOTO, Kotaro; INOKUMA, Hisashi; KAWAZU, Shin-Ichiro; IGARASHI, Ikuo; XUAN, Xuenan; YOKOYAMA, Naoaki; 松本, 高太郎; 猪熊, 壽; 河津, 信一郎; 五十嵐, 郁男; 玄, 学南; 横山, 直明. |
| Theileria orientalis is a benign bovine protozoan parasite that occasionally causes serious economic loss in the livestock industry. We report the findings of a molecular epidemiological survey of T. orientalis in 94 Vietnamese yellow cattle, 43 water buffaloes, 21 sheep, 21 goats and 85 blood-sucking ticks of cattle in the Thua Thien Hue province of Vietnam. The major piroplasm surface protein (MPSP) gene of T. orientalis was detected using polymerase chain reaction from 13 cattle (13.8%), 11 water buffaloes (25.6%), 1 sheep (4.8%) and 9 ticks (10.6%). Phylogenetic analysis using MPSP gene sequences showed the presence of seven genotypes, four previously categorized genotypes (Types 1, 3, 5 and 7) and three new genotypes (Types N-1, N-2 and N-3). |
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Palavras-chave: Cattle; Genotype; MPSP; Theileria orientalis; Vietnam. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3585 |
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| CHIBA, Shiori; FUNATO, Shingo; HORIUCHI, Noriyuki; MATSUMOTO, Kotaro; INOKUMA, Hisashi; FURUOKA, Hidefumi; KOBAYASHI, Yoshiyasu; 堀内, 雅之; 松本, 高太郎; 猪熊, 壽; 古岡, 秀文; 古林, 与志安. |
| Degeneration of the optic pathway has been reported in various animal species including cattle. We experienced a case of bilateral optic tract degeneration characterized by severe gliosis in a Japanese black cattle without any obvious visual defects. To evaluate the significance, pathological nature and pathogenesis of the lesions, we examined the optic pathway in 60 cattle (41 Japanese black, 13 Holstein and 6 crossbreed) with or without ocular abnormalities. None of these animals had optic canal stenosis. Degenerative changes with severe gliosis in the optic pathway, which includes the optic nerve, optic chiasm and optic tract, were only observed in 8 Japanese black cattle with or without ocular abnormalities. Furthermore, strong immunoreactivity of... |
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Palavras-chave: Axonal degeneration; Gliosis; Japanese black cattle; Optic pathway degeneration. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4123 |
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| TAGAWA, Michihito; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| Hemotropic mycoplasmas (hemoplasmas) are cell-wall deficient, epierythrocytic bacteria that cause infectious anemia in several mammalian species. The prevalence of hemoplasma species was examined by screening and species-specific PCR using blood samples collected from 51 sika deer in Hokkaido, Japan. Molecular analyses were performed for the 16S rRNA, 23S rRNA and RNase P RNA (rnpB) gene sequences. A total of 23/51 (45%) deer DNA samples were positive for hemoplasmas in the screening PCR. Using species-specific PCR, 12 and 17 samples were positive for ‘Candidatus Mycoplasma haemocervae’ and ‘Candidatus M. erythrocervae’, respectively. Sequencing and phylogenetic trees of those three genes indicate that the ‘Candidatus M. haemocervae’ and ‘Candidatus M.... |
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Palavras-chave: Cervus nippon yesoensis; Hemoplasma; Japan; Phylogeny. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4012 |
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| TAGAWA, Michihito; YBANEZ, Adrian P.; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| The prevalence and risk factors of 2 bovine hemoplasma species—Mycoplasma wenyonii (Mw) and “Candidatus Mycoplasma haemobos” (CMh)—were examined by direct PCR using whole blood samples collected from 343 cattle in Eastern Hokkaido, Japan. The sensitivity of the direct PCR assay was 10-fold higher than the standard PCR assay, detecting 5 copies of bovine hemoplasma DNA per reaction. An epidemiological survey of sampling location, living conditions, age and bovine leukemia virus antibody was performed to assess infection risk factors. The prevalence rates were 38.5% for Mw and 39.1% for CMh, respectively, with an overall prevalence rate of 64.7% for all bovine hemoplasma infections. Significant differences were found in sampling location, living conditions... |
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Palavras-chave: Bovine hemoplasma; Direct PCR; Japan; Risk factor. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3838 |
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| YBAÑEZ, Adrian Patalinghug; SIVAKUMAR, Thillaiampalam; BATTSETSEG, Badgar; BATTUR, Banzragch; ALTANGEREL, Khukhuu; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| Anaplasma marginale is an etiologic agent of bovine anaplasmosis. This study aimed to molecularly detect and characterize A. marginale that is prevalent in Mongolian cattle populations. A highly specific and sensitive nested PCR (nPCR) method based on the Msp5 gene was developed to detect A. marginale (Msp5 nPCR). The method detected A. marginale from the positive DNA samples obtained from different countries, while no amplicons were observed from DNA samples of several other bovine blood pathogens tested. The detection limit of Msp5 nPCR was determined to be 2 copies/μl. The method was tested against field blood DNA samples prepared from 300 Mongolian cattle in 2010. Results indicated a prevalence rate of 8.7% (26 of 300). On the other hand, partial DNA... |
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Palavras-chave: Anaplasma marginale; Cattle; Mongolia; Msp5; PCR. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3867 |
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| YBAÑEZ, Adrian Patalinghug; SATO, Fumio; NAMBO, Yasuo; FUKUI, Takashi; MASUZAWA, Toshiyuki; OHASHI, Norio; MATSUMOTO, Kotaro; KISHIMOTO, Toshio; INOKUMA, Hisashi; 松本, 高太郎; 猪熊, 壽. |
| A total of 87 Thoroughbred horses and 10 ixodid ticks from a ranch in Hidaka district, Hokkaido were tested for tick-borne diseases. Using the indirect fluorescent antibody (IFA) method, 3.4, 92.0 and 97.7% of the horses showed antibody titers of ≥80 against Anaplasma phagocytophilum, Rickettsia helvetica, and Borrelia garinii, respectively. This is the first report of infection with the 3 pathogens in horses in Japan. Using PCR, DNAs from the peripheral blood of all horses were found negative with any Anaplasma, Rickettsia and Borrelia spp., while those from Haemaphysalis megaspinosa ticks were found positive for Anaplasma sp. closely related to A. phagocytophilum in Japan, and A. bovis. B. japonica was also detected in an H. flava tick for the first time. |
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Palavras-chave: Anaplasma sp.; Borrelia sp.; Rickettsia sp.; Thoroughbred horse; Tick-borne diseases. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3840 |
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| LEE, Ki-Ja; KISHIMOTO, Miori; SHIMIZU, Junichiro; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; SASAKI, Naoki; ISHII, Mitsuo; INOKUMA, Hisashi; IWASAKI, Toshiroh; MIYAKE, Yoh-Ichi; YAMADA, Kazutaka; 古林, 与志安; 松本, 高太郎; 佐々木, 直樹; 石井, 三都夫; 猪熊, 壽; 山田, 一孝. |
| We report here the non-contrast and contrast-enhanced computed tomography performed in two calves with brain abscess and multiple pulmonary abscesses with pharyngeal abscess, respectively. Contrast-enhanced computed tomography was useful in the diagnosis of these diseases in both calves. The diseases were confirmed by histopathological examination. |
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Palavras-chave: Abscess; Cattle; Contrast-enhanced CT; Diagnosis. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3891 |
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| 吉本, 薫; 駒形, 真; 千葉, 史織; 弘, 雅信; 松本, 高太郎; 古林, 与志安; 猪熊, 壽; YOSHIMOTO, Kaoru; KOMAGATA, Makoto; CHIBA, Shiori; HIRO, Masahiro; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; INOKUMA, Hisashi. |
| 5歳6カ月齢のホルスタイン種乳牛が鼻出血，眼球突出および黒色便を呈したため，地方病性牛白血病を疑った．し かし，生前の臨床検査では牛白血病の確定診断はできず，病理組織学的検査により鼻腔内骨肉腫と診断された．本症例では，明瞭な呼吸器症状や顔面の変形といった鼻腔内腫瘍を疑わせる所見が乏しく，生前診断が困難であった． Enzootic bovine leukosis was suspected in a Holstein cow aged 5-years and 6-months, as it displayed symptoms of epistaxis, proptosis, and melena. However, clinicopathological findings failed to confirm a diagnosis of bovine leukosis, and histopathological findings revealed a nasal osteosarcoma. As the case lacked typical findings of nasal tumor such as sneezing and facial deformity, a nasal tumor diagnosis would have been difficult. |
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Palavras-chave: ホルスタイン; 鼻腔内腫瘍; 骨肉腫; Holstein; Nasal tumor; Osteosarcoma. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3554 |
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| Registros recuperados: 31 | |
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